Not known Factual Statements About cytometer
Not known Factual Statements About cytometer
Blog Article
At that time, the early indicators have been dismissed as particles, nevertheless the retrospective evaluation shown shared attributes with the problematic cells in tradition. Tracing the an infection served the lab create additional laboratory treatments for screening and defense of assay-vital mobile traces.
As samples are acquired around the Attune CytPix Flow Cytometer, the high-speed brightfield digital camera captures and retailers visuals of detected activities in a charge up to six,000 photos per next, based on the flow price and graphic size. For increased versatility, Attune Cytometric Program helps you to change the graphic capture frequency as wanted and choose a specific gate for image capture, if essential.
Incorporate acoustic concentrating technology with higher-parameter Examination for versatile spectral unmixing and conventional workflows.
Find new insights into your experimental details by visualizing populations working with combined fluorescence and morphology data. Our software interface wizards allow downsampling, concatenation (merged files) and dimensionality instruments which can be built into the computer software for an intuitive, streamlined information Evaluation practical experience.
"A picture is not really value 1000 dots, nevertheless it truly really helps to see photographs. It is great to check out visuals that correlate with staining. By way of example, AnnexinV-constructive cells which might be morphologically distinctive than balanced cells, or CD14+ monocytes which have been more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it is actually illuminating to determine the amount of doublets slip through standard singlet gates, and wonderful to confirm the position of unexpected double-beneficial gatherings in my Evaluation."
Pair your Attune flow cytometer with an autosampler for improved workflow performance, and include robotic integration for maximum throughput.
Our experts will design and style panels in your case as persistently you will need—free of charge, no merchandise buy needed.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can count on for reliable and trustworthy functionality. The Attune CytPix design features a high-pace brightfield digicam and AI-driven impression Investigation application, with new updates which includes machine Finding out tools and an intuitive person interface, enabling Increased details precision, precision, and more rapidly insights into sample biology.
People can check out function pictures in authentic-time to verify sample top quality, optimize protocols, and history the morphology of each cell populace recognized for Investigation. Imaging seize also Positive aspects from acoustic focusing, which helps to place the cells so that a sharp, centered graphic is attained.
Audio waves help precise cell alignment, resulting in Extraordinary data website high quality and ~10X speedier sample flow rates.
We Formerly shown the strength of imaging Motor vehicle-T/Ramos cell interactions. Allow’s look at just the inhabitants of finest fascination, the double constructive functions, To find out more. We can now use extended morphological parameters (right here, circularity and skewness of depth) to additional analyze the features of those populations and refine gating on these situations, increasing details robustness.
Constructed-in dimensionality reduction tools increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate predicted populace resolution in lysed full human blood. Person can watch activities from Just about every cluster for visual confirmation of mobile phenotypes.
No clean/no lyse leukocyte analysis with imaging. Cells have been obtained from 24-hour-aged blood by dilution in one mM EDTA ( Pro UX/UI allows people to practice AI styles for expanded programs. (A) The “Prepare Product” feature provides annotation applications and actual-time model coaching accuracy.
At the heart of each Attune Flow Cytometer lies our groundbreaking acoustic focusing technological innovation, which utilizes audio waves to meticulously align cells in the flow mobile. This innovative strategy surpasses standard hydrodynamic methods, leading to speedier processing speeds, exceptional details high quality, and greater sample throughput.
Because the cell line experienced previously been analyzed within the Attune CytPix Flow Cytometer, the researchers went back to the pictures and ended up capable of document the microbial an infection at the least five times previously.
Agilent Worth Promise ensures you no less than ten years of instrument use from your date you buy. In any other case, We are going to credit score you with the residual price of the program toward an upgraded design.
What seemed to be a homogeneous inhabitants is actually additional various—an Perception that ought to be viewed as when interpreting outcomes.